Gene technology tools and methods — A/L Biology

How do I practise Gene technology tools and methods?

1 question with a worked solution

1. Which enzyme is used in recombinant DNA technology to catalyze the formation of phosphodiester bonds to join foreign DNA fragments into a plasmid vector?
   1. A) DNA ligase
   2. B) Restriction endonuclease
   3. C) Reverse transcriptase
   4. D) DNA polymerase
   Check my answer

   Correct answer: A) DNA ligase

   DNA ligase catalyzes the synthesis of phosphodiester bonds between the 3'-hydroxyl and 5'-phosphate ends of adjacent nucleotides, sealing nicks and joining DNA fragments.

   Show worked solution

   Step 1: Identify molecular tools in cloning: - Restriction enzymes cut DNA at specific palindromic sequences. - DNA ligase joins/seals DNA fragments. - DNA polymerase synthesizes new DNA strands. Step 2: Joining fragments into vectors requires DNA ligase. Answer: DNA ligase.
   Modelled on: the A/L Biology examination pattern

Get this auto-marked

Next in the syllabus

Where does Gene technology tools and methods fit in the syllabus?

Year 1 · Term 2