Applications of gene technology — A/L Biology

How do I practise Applications of gene technology?

1 question with a worked solution

1. In human insulin production via recombinant DNA technology, which bacterium is most widely utilized as the host organism to express insulin polypeptides?
   1. A) Escherichia coli
   2. B) Bacillus thuringiensis
   3. C) Agrobacterium tumefaciens
   4. D) Streptomyces griseus
   Check my answer

   Correct answer: A) Escherichia coli

   Escherichia coli is the standard bacterial host organism engineered to express the A and B chains of human insulin, which are subsequently extracted and linked by disulfide bonds.

   Show worked solution

   Step 1: Recall industrial recombinant proteins: Humulin was first produced commercially using E. coli. Step 2: Bacillus thuringiensis produces Bt toxin; Agrobacterium transforms plants. Step 3: E. coli is the bacterial expression system for recombinant human insulin. Answer: Escherichia coli.
   Modelled on: the A/L Biology examination pattern

Get this auto-marked

Next in the syllabus

Where does Applications of gene technology fit in the syllabus?

Year 1 · Term 2