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Recycling of solid wastes — A/L Biology

Aligned with the NIE syllabus

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1 question with a worked solution

  1. During the anaerobic biomethanation of organic municipal solid waste in a commercial biodigester, which microbial group performs the terminal biochemical step converting acetate, hydrogen, and carbon dioxide into methane gas?
    1. A) Acetogenic sulfate-reducing bacteria
    2. B) Acidogenic fermentative bacteria
    3. C) Methanogenic archaea (Methanogens)
    4. D) Hydrolytic cellulolytic fungi
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    Correct answer: C) Methanogenic archaea (Methanogens)

    Anaerobic digestion proceeds through four stages: hydrolysis, acidogenesis, acetogenesis, and methanogenesis. In the final stage, obligate anaerobic Methanogenic archaea (e.g., Methanobacterium, Methanosarcina) reduce acetate (acetoclastic methanogenesis) or CO₂ + H₂ (hydrogenotrophic methanogenesis) to produce combustible CH₄ and CO₂ (biogas).

    Show worked solution
    Step 1: Trace stages of anaerobic digestion: 1. Hydrolysis (polymers -> monomers by hydrolytic bacteria) 2. Acidogenesis (monomers -> volatile fatty acids) 3. Acetogenesis (fatty acids -> acetic acid, CO₂, H₂) 4. Methanogenesis (acetate/CO₂/H₂ -> CH₄ + CO₂ by Methanogens) Answer: Methanogenic archaea (Methanogens).
    Modelled on: the A/L Biology examination pattern

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Year 2 · Term 3

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